Optimizing Premature Chromosome Condensation (PCC) of Human Lymphocytes by Somatic Cell Hybridization to Study Primary DNA Damages
نویسندگان
چکیده
منابع مشابه
Induction of premature chromosome condensation (PCC) by calyculin A for biodosimetry
Background: Premature chromosome condensation (PCC) is a method for interphase chromosome analysis in biodosimetry. This study was performed to verify the usefulness of PCC induced by calyculin A in human peripheral blood lymphocytes (pbl) for biological dosimetry and possible construction of dose-response curve. Materials and Methods: Peripheral blood was obtained from a healthy donor and expo...
متن کاملBiological Dose Assessment by Chemical Induction of Premature Chromosome Condensation
Dose assessment of radiation victims is a key element in medical management of radiation accidents. At high radiation doses (> 6 Gy), dicentric assay lose its efficiency and premature chromosome condensation (PCC) assay was proposed to overcome the restrictions of dicentric assay. It was shown that PCC ring is more suitable and simpler than dicentric for biodosimetry in high radiation doses. In...
متن کاملinduction of premature chromosome condensation (pcc) by calyculin a for biodosimetry
background: premature chromosome condensation (pcc) is a method for interphase chromosome analysis in biodosimetry. this study was performed to verify the usefulness of pcc induced by calyculin a in human peripheral blood lymphocytes (pbl) for biological dosimetry and possible construction of dose-response curve. materials and methods: peripheral blood was obtained from a healthy donor and expo...
متن کاملPremature chromosome condensation in human leukemia.
Premature chromosome condensation (PCC) has previously been observed in tissue culture and is believed to arise from asynchronous mitotic activity in multinucleated cells in which the affected nucleus is in interphase and at least one nucleus is in metaphase. Such cells have been noted following fusion induced by virus infection, spontaneously, and after treatment with cytochalasin B. The pheno...
متن کاملInduction of Premature Chromosome Condensation (PCC) by Calyculin A for biodosimetry
B iological dosimetry of ionizing radiation is generally performed by scoring dicentrics and/or translocations in chromosome from peripheral blood lymphocytes (pbl) at the first mitosis following in vitro growth stimulation. It is well known that after irradiation, in this procedure mitotic index is low in some individuals, so the scored mitoses might not be representative of the exposed cell p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: INTERNATIONAL JOURNAL OF HUMAN GENETICS
سال: 2007
ISSN: 0972-3757,2456-6330
DOI: 10.31901/24566330.2007/07.04.08